Difference between revisions of "YsxC"

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(Original publications)
(Original publications)
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<pubmed> 19575570 </pubmed>
 
<pubmed> 19575570 </pubmed>
 
==Original publications==
 
==Original publications==
<pubmed>17981968,11073929 ,12427945, 16997968 7961402 9852015 14684918 </pubmed>
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<pubmed>17981968,11073929 ,12427945, 16997968 7961402 9852015 14684918 15136032</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:13, 16 June 2010

  • Description: assembly of the 50S subunit of the ribosome

Gene name ysxC
Synonyms orfX
Essential yes PubMed
Product GTPase
Function ribosome assembly
Metabolic function and regulation of this protein in SubtiPathways:
Stress
MW, pI 21 kDa, 9.708
Gene length, protein length 585 bp, 195 aa
Immediate neighbours ysxD, lonA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YsxC context.gif







The gene

Basic information

  • Locus tag: BSU28190

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: engB family (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 1SVI (complex with GDP), 1SUL
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP834, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP842, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)

Original publications

Catherine Wicker-Planquart, Anne-Emmanuelle Foucher, Mathilde Louwagie, Robert A Britton, Jean-Michel Jault
Interactions of an essential Bacillus subtilis GTPase, YsxC, with ribosomes.
J Bacteriol: 2008, 190(2);681-90
[PubMed:17981968] [WorldCat.org] [DOI] (I p)

Laura Schaefer, William C Uicker, Catherine Wicker-Planquart, Anne-Emmanuelle Foucher, Jean-Michel Jault, Robert A Britton
Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis.
J Bacteriol: 2006, 188(23);8252-8
[PubMed:16997968] [WorldCat.org] [DOI] (P p)

Sergey N Ruzheinikov, Sanjan K Das, Svetlana E Sedelnikova, Patrick J Baker, Peter J Artymiuk, Jorge García-Lara, Simon J Foster, David W Rice
Analysis of the open and closed conformations of the GTP-binding protein YsxC from Bacillus subtilis.
J Mol Biol: 2004, 339(2);265-78
[PubMed:15136032] [WorldCat.org] [DOI] (P p)

Sanjan K Das, Svetlana E Sedelnikova, Patrick J Baker, Sergey N Ruzheinikov, Simon J Foster, David W Rice
Expression, purification, crystallization and preliminary crystallographic analysis of a putative GTP-binding protein, YsxC, from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2004, 60(Pt 1);166-8
[PubMed:14684918] [WorldCat.org] [DOI] (P p)

Takuya Morimoto, Pek Chin Loh, Tomohiro Hirai, Kei Asai, Kazuo Kobayashi, Shigeki Moriya, Naotake Ogasawara
Six GTP-binding proteins of the Era/Obg family are essential for cell growth in Bacillus subtilis.
Microbiology (Reading): 2002, 148(Pt 11);3539-3552
[PubMed:12427945] [WorldCat.org] [DOI] (P p)

Z Prágai, C R Harwood
YsxC, a putative GTP-binding protein essential for growth of Bacillus subtilis 168.
J Bacteriol: 2000, 182(23);6819-23
[PubMed:11073929] [WorldCat.org] [DOI] (P p)

E Krüger, M Hecker
The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes.
J Bacteriol: 1998, 180(24);6681-8
[PubMed:9852015] [WorldCat.org] [DOI] (P p)

S Riethdorf, U Völker, U Gerth, A Winkler, S Engelmann, M Hecker
Cloning, nucleotide sequence, and expression of the Bacillus subtilis lon gene.
J Bacteriol: 1994, 176(21);6518-27
[PubMed:7961402] [WorldCat.org] [DOI] (P p)