Difference between revisions of "PGP380"

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[[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']]
 
[[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']]
  
Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the [[SPINE]] technology
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The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is based on the vectors [[pBQ200]] and [[pHT315]].
 
 
constructed in [[Stülke]] lab
 
 
 
in ''E. coli'': ampicillin resistance
 
 
 
in ''B. subtilis'': erythromycin resistance
 
 
 
based on [[pBQ200]]
 
 
 
similar vector: [[pGP382]]
 
  
 
sequencing primer:  
 
sequencing primer:  

Revision as of 11:47, 25 February 2010

pGP380: click to enlarge
pGP380 cloning region: click to enlarge

The vector was constructed in the Stülke lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pGP380 can be used for the SPINE method. pGP382 is similar to the vector pGP380 which is based on the vectors pBQ200 and pHT315.

sequencing primer:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘