Difference between revisions of "SpoIIM"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''spoIIM'' {{PubMed|8501065}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|8501065}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|8501065}}
 +
** subject to negative control by [[YlbO]] {{PubMed|15383836}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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=References=
 
=References=
  
<pubmed>17376078,11886548,15752199 , </pubmed>
+
<pubmed>17376078,11886548,15752199 ,8501065 15383836  </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:57, 18 November 2009

  • Description: required for dissolution of the septal cell wall

Gene name spoIIM
Synonyms
Essential no
Product sporulation protein
Function dissolution of the septal cell wall
MW, pI 24 kDa, 9.84
Gene length, protein length 642 bp, 214 aa
Immediate neighbours fur, yqkK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIIM context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU23530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed early during sporulation in the mother cell (SigE) PubMed
    • subject to negative control by YlbO PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Arnaud Chastanet, Richard Losick
Engulfment during sporulation in Bacillus subtilis is governed by a multi-protein complex containing tandemly acting autolysins.
Mol Microbiol: 2007, 64(1);139-52
[PubMed:17376078] [WorldCat.org] [DOI] (P p)

Thierry Doan, Kathleen A Marquis, David Z Rudner
Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.
Mol Microbiol: 2005, 55(6);1767-81
[PubMed:15752199] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

P Eichenberger, P Fawcett, R Losick
A three-protein inhibitor of polar septation during sporulation in Bacillus subtilis.
Mol Microbiol: 2001, 42(5);1147-62
[PubMed:11886548] [WorldCat.org] [DOI] (P p)

K Smith, P Youngman
Evidence that the spoIIM gene of Bacillus subtilis is transcribed by RNA polymerase associated with sigma E.
J Bacteriol: 1993, 175(11);3618-27
[PubMed:8501065] [WorldCat.org] [DOI] (P p)