Difference between revisions of "MalK"

From SubtiWiki
Jump to: navigation, search
Line 84: Line 84:
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05250 O05250]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05250 O05250]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU31520]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU31520]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 09:49, 25 June 2009

  • Description: two-component sensor kinase, regulation of malate uptake

Gene name malK
Synonyms yufL
Essential no
Product two-component sensor kinase
Function regulation of malate uptake
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 58 kDa, 6.947
Gene length, protein length 1599 bp, 533 aa
Immediate neighbours yufK, malR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YufL context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU31520

Phenotypes of a mutant

unable to use malate as the single carbon source PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot) sensor kinase
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: two transmembrane segments, C-terminal histidine phosphotransferase domain
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References

Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading): 2003, 149(Pt 9);2331-2343
[PubMed:12949160] [WorldCat.org] [DOI] (P p)

Kousei Tanaka, Kazuo Kobayashi, Naotake Ogasawara
The Bacillus subtilis YufLM two-component system regulates the expression of the malate transporters MaeN (YufR) and YflS, and is essential for utilization of malate in minimal medium.
Microbiology (Reading): 2003, 149(Pt 9);2317-2329
[PubMed:12949159] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)