Difference between revisions of "ThiM"

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# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 21:54, 13 June 2009

  • Description: hydroxyethylthiazole kinase

Gene name thiM
Synonyms thiK, ipa-25d, ywbJ
Essential no
Product hydroxyethylthiazole kinase
Function biosynthesis of thiamine
MW, pI 28 kDa, 5.393
Gene length, protein length 816 bp, 272 aa
Immediate neighbours thiE, ywbI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiM context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU38300

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole (according to Swiss-Prot)
  • Protein family: Thz kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

N Campobasso, I I Mathews, T P Begley, S E Ealick
Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution.
Biochemistry: 2000, 39(27);7868-77
[PubMed:10891066] [WorldCat.org] [DOI] (P p)

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed