Difference between revisions of "RplU"

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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 306 bp, 102 aa  
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 306 bp, 102 aa  
 
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ysxB]]'', ''[[spoIVFB]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[prp]]'', ''[[spoIVFB]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27960 DNA_with_flanks]
 
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27960 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27960 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27960 DNA_with_flanks]

Revision as of 12:42, 10 December 2014

Gene name rplU
Synonyms
Essential yes PubMed
Product ribosomal protein L21 (BL20)
Function translation
Gene expression levels in SubtiExpress: rplU
Interactions involving this protein in SubtInteract: RplU
MW, pI 11 kDa, 10.16
Gene length, protein length 306 bp, 102 aa
Immediate neighbours prp, spoIVFB
Sequences Protein DNA DNA_with_flanks
Genetic context
RplU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RplU expression.png















Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU27960

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 5041 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 21010 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)