Difference between revisions of "ArgS"
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 1206 {{PubMed|24696501}} | ** number of protein molecules per cell (minimal medium with glucose and ammonium): 1206 {{PubMed|24696501}} | ||
** number of protein molecules per cell (complex medium with amino acids, without glucose): 3289 {{PubMed|24696501}} | ** number of protein molecules per cell (complex medium with amino acids, without glucose): 3289 {{PubMed|24696501}} | ||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1716 {{PubMed|21395229}} | ||
+ | |||
+ | ** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3749 {{PubMed|21395229}} | ||
=Biological materials = | =Biological materials = | ||
− | |||
* '''Mutant:''' | * '''Mutant:''' | ||
Revision as of 13:32, 17 April 2014
- Description: arginyl-tRNA synthetase, universally conserved protein
Gene name | argS |
Synonyms | |
Essential | yes PubMed |
Product | arginyl-tRNA synthetase |
Function | translation |
Gene expression levels in SubtiExpress: argS | |
Metabolic function and regulation of this protein in SubtiPathways: argS | |
MW, pI | 62 kDa, 5.11 |
Gene length, protein length | 1668 bp, 556 aa |
Immediate neighbours | narK, ywiB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
translation, essential genes, universally conserved proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37330
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU37330
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg) (according to Swiss-Prot)
- Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU37330
- Structure: 3FNR (from Campylobacter jejuni, 39% identity, 56% similarity)
- UniProt: P46906
- KEGG entry: [3]
- E.C. number: 6.1.1.19
Additional information
Expression and regulation
- Operon: argS PubMed
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
- number of protein molecules per cell (minimal medium with glucose and ammonium): 1206 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 3289 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1716 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 3749 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933]
[WorldCat.org]
[DOI]
(P p)