Difference between revisions of "PurN"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 146 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 10:01, 17 April 2014

  • Description: phosphoribosylglycinamide formyltransferase, irreversible

Gene name purN
Synonyms
Essential no
Product phosphoribosylglycinamide formyltransferase
Function purine biosynthesis
Gene expression levels in SubtiExpress: purN
Metabolic function and regulation of this protein in SubtiPathways:
purN
MW, pI 21 kDa, 6.182
Gene length, protein length 585 bp, 195 aa
Immediate neighbours purM, purH
Sequences Protein DNA DNA_with_flanks
Genetic context
PurN context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurN expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

G-box, PurR regulon

The gene

Basic information

  • Locus tag: BSU06510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide (according to Swiss-Prot)
  • Protein family: GART family (according to Swiss-Prot)
  • Paralogous protein(s): YkkE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3P9X (from B. halodurans, 51% identity, 70% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
    • repressed in the presence of guanine(G-box) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 146 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References