Difference between revisions of "GuaB"
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{{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}}, | {{SubtiWiki category|[[biosynthesis/ acquisition of nucleotides]]}}, | ||
{{SubtiWiki category|[[essential genes]]}}, | {{SubtiWiki category|[[essential genes]]}}, | ||
| − | {{SubtiWiki category|[[phosphoproteins]]}} | + | {{SubtiWiki category|[[phosphoproteins]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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** the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed] | ** the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed] | ||
** inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´{{PubMed|6111556}} | ** inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´{{PubMed|6111556}} | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
| − | <pubmed>22938038,17611193,12884008,1722815, 22981860,12618455,17726680 17726680 6111556 24163341</pubmed> | + | <pubmed>22938038,17611193,12884008,1722815, 22981860,12618455,17726680 17726680 6111556 24163341 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 16:01, 5 March 2014
- Description: IMP dehydrogenase
| Gene name | guaB |
| Synonyms | guaA |
| Essential | yes PubMed |
| Product | IMP dehydrogenase |
| Function | biosynthesis of GMP |
| Gene expression levels in SubtiExpress: GuaB | |
| Metabolic function and regulation of this protein in SubtiPathways: guaB | |
| MW, pI | 52 kDa, 6.168 |
| Gene length, protein length | 1464 bp, 488 aa |
| Immediate neighbours | yaaC, dacA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides, essential genes, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00090
Phenotypes of a mutant
- essential PubMed
- reduced expression of guaB suppresses the requirement of a relA sasA sasB triple mutant for branched chain amino acids, methionine and threonine PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH (according to Swiss-Prot)
- Protein family: IMPDH/GMPR family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed
Database entries
- Structure: 1VRD (from Thermotoga maritima msb8, 60% identity, 80% similarity)
- UniProt: P21879
- KEGG entry: [3]
- E.C. number: 1.1.1.205
Additional information
Expression and regulation
- Operon: guaB PubMed
- Additional information:
- the mRNA is very stable (half-life > 15 min) PubMed
- inhibition of enzymatic activity by (p)ppGpp during the ´stringent response´PubMed
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
