Difference between revisions of "EpsB"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' GP1518 (aphA3), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 16:36, 27 January 2014

  • Description: extracellular polysaccharide synthesis, putative protein tyrosine kinase

Gene name epsB
Synonyms yveL
Essential no
Product unknown
Function biofilm formation
Gene expression levels in SubtiExpress: epsB
Interactions involving this protein in SubtInteract: EpsB
Regulation of this protein in SubtiPathways:
epsB
MW, pI 24 kDa, 9.918
Gene length, protein length 681 bp, 227 aa
Immediate neighbours epsC, epsA
Sequences Protein DNA DNA_with_flanks
Genetic context
YveL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
EpsB expression.png















Categories containing this gene/protein

protein modification, biofilm formation

This gene is a member of the following regulons

AbrB regulon, RemA regulon, SinR regulon

The gene

Basic information

  • Locus tag: BSU34360

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot)
  • Paralogous protein(s): PtkA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2VED (CapB, the homolog in Staphylococcus aureus) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

Biological materials

  • Mutant: GP1518 (aphA3), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:
  • FLAG-tag construct: GP1541 (epsB-3xFLAG spc trpC2) , available in Jörg Stülke's lab

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)


Original publications