Difference between revisions of "WapA"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
Line 59: Line 59:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU39230&redirect=T BSU39230]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/wapA-yxxG.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/wapA-yxxG.html]
Line 100: Line 101:
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU39230&redirect=T BSU39230]
  
 
* '''Structure:'''
 
* '''Structure:'''

Revision as of 15:11, 2 April 2014

  • Description: cell wall-associated protein precursor, contact-dependent growth inhibition protein

Gene name wapA
Synonyms
Essential no
Product cell wall-associated protein precursor
Function intercellular competition
Gene expression levels in SubtiExpress: wapA
Interactions involving this protein in SubtInteract: WapA
MW, pI 258 kDa, 6.131
Gene length, protein length 7002 bp, 2334 aa
Immediate neighbours yxxG, yxxF
Sequences Protein DNA DNA_with_flanks
Genetic context
WapA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
WapA expression.png















Categories containing this gene/protein

cell wall/ other, toxins, antitoxins and immunity against toxins

This gene is a member of the following regulons

DegU regulon, WalR regulon, YvrHb regulon

The gene

Basic information

  • Locus tag: BSU39230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relexed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • the C-terminal toxic domain has RNase activity (cleaves tRNAs) PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • cell wall binding domain as retention signal
    • C-terminal toxic domain with RNase activity (cleaves tRNAs) PubMed
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • extracellular (signal peptide), cell wall binding domain as retention signal, major constituent of the secretome PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed at high salt concentration (DegU-P) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References