Difference between revisions of "MotB"

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(Phenotypes of a mutant)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
* loss of swimming and swarming motility {{PubMed|24296669}}
 
* loss of swimming and swarming motility {{PubMed|24296669}}
* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669}}
+
* mucoid phenotype due to the [[DegU]]-P activated overexpression of the ''[[capB]]-[[capC]]-[[capA]]-[[capE]]'' operon and resulting overproduction of poly-gamma-glutamate {{PubMed|24296669,23888912}}
  
 
=== Database entries ===
 
=== Database entries ===
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' available in [[Nicola Stanley-Wall]]'s lab {{PubMed|23888912}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
<pubmed>6313226 24296669</pubmed>
+
<pubmed>6313226 24296669 23888912</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:30, 16 February 2014

  • Description: H+-coupled MotA-MotB flagellar stator

Gene name motB
Synonyms mot
Essential no
Product motility protein (flagellar motor rotation)
Function motility and chemotaxis
Gene expression levels in SubtiExpress: motB
Interactions involving this protein in SubtInteract: MotB
MW, pI 29 kDa, 6.652
Gene length, protein length 783 bp, 261 aa
Immediate neighbours mhqR, motA
Sequences Protein DNA DNA_with_flanks
Genetic context
MotB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MotB expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU13680

Phenotypes of a mutant

  • loss of swimming and swarming motility PubMed
  • mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: OmpA-like domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J Bacteriol: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Lynne S Cairns, Victoria L Marlow, Emma Bissett, Adam Ostrowski, Nicola R Stanley-Wall
A mechanical signal transmitted by the flagellum controls signalling in Bacillus subtilis.
Mol Microbiol: 2013, 90(1);6-21
[PubMed:23888912] [WorldCat.org] [DOI] (I p)

M Z Gilman, M J Chamberlin
Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
Cell: 1983, 35(1);285-93
[PubMed:6313226] [WorldCat.org] [DOI] (P p)