Difference between revisions of "DgcW"

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(Biological materials)
(Biological materials)
Line 121: Line 121:
 
** GP850 (''[[ykoW]]''::''cat''), available in [[Jörg Stülke]]'s lab
 
** GP850 (''[[ykoW]]''::''cat''), available in [[Jörg Stülke]]'s lab
 
** BP140 (''[[ykoW]]''::''ermC'') available in [[Fabian Commichau]]'s lab
 
** BP140 (''[[ykoW]]''::''ermC'') available in [[Fabian Commichau]]'s lab
 +
** BP142 (''[[ykoWVU]]''::''kan'') available in [[Fabian Commichau]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:40, 6 August 2013

  • Description: potential diguanylate cyclase and phosphodiesterase

Gene name ykoW
Synonyms
Essential no
Product potential diguanylate cyclase and phosphodiesterase
Function unknown
Gene expression levels in SubtiExpress: ykoW
MW, pI 84 kDa, 6.823
Gene length, protein length 2247 bp, 749 aa
Immediate neighbours ykoV, ykoX
Sequences Protein DNA DNA_with_flanks
Genetic context
YkoW context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkoW expression.png















Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU13420

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • contains a central GGDEF domain and a C-terminal EAL domain PubMed
    • aa 290-735 are similar to E. coli CsrD (18% identity, 43% similarity)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References