Difference between revisions of "HxlB"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tlpC]]'', ''[[hxlA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tlpC]]'', ''[[hxlA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03450 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU03450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU03450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU03450 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:hxlB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:hxlB_context.gif]]

Revision as of 09:27, 14 May 2013

  • Description: 6-phospho-3-hexuloisomerase

Gene name hxlB
Synonyms yckF
Essential no
Product 6-phospho-3-hexuloisomerase
Function ribulose monophosphate pathway for formaldehyde fixation
Gene expression levels in SubtiExpress: hxlB
MW, pI 19 kDa, 5.791
Gene length, protein length 555 bp, 185 aa
Immediate neighbours tlpC, hxlA
Sequences Protein DNA DNA_with_flanks
Genetic context
HxlB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
HxlB expression.png
























Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HxlR regulon

The gene

Basic information

  • Locus tag: BSU03450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-arabino-hex-3-ulose 6-phosphate = D-fructose 6-phosphate (according to Swiss-Prot)
  • Protein family: PHI subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: induction by formaldehyde (HxlR) PubMed
  • Regulatory mechanism: transcription activation by HxlR PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Thi Thu Huyen Nguyen, Warawan Eiamphungporn, Ulrike Mäder, Manuel Liebeke, Michael Lalk, Michael Hecker, John D Helmann, Haike Antelmann
Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR).
Mol Microbiol: 2009, 71(4);876-94
[PubMed:19170879] [WorldCat.org] [DOI] (I p)

Nobuo Kato, Hiroya Yurimoto, Rudolf K Thauer
The physiological role of the ribulose monophosphate pathway in bacteria and archaea.
Biosci Biotechnol Biochem: 2006, 70(1);10-21
[PubMed:16428816] [WorldCat.org] [DOI] (P p)

E J Taylor, S J Charnock, J Colby, G J Davies, G W Black
Cloning, purification and characterization of the 6-phospho-3-hexulose isomerase YckF from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2001, 57(Pt 8);1138-40
[PubMed:11468398] [WorldCat.org] [DOI] (P p)

H Yasueda, Y Kawahara, S Sugimoto
Bacillus subtilis yckG and yckF encode two key enzymes of the ribulose monophosphate pathway used by methylotrophs, and yckH is required for their expression.
J Bacteriol: 1999, 181(23);7154-60
[PubMed:10572115] [WorldCat.org] [DOI] (P p)