Difference between revisions of "RapJ"
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* '''Domains:''' | * '''Domains:''' | ||
− | + | ** RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide [[PhrC]], the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. {{PubMed|23526881}} | |
* '''Modification:''' | * '''Modification:''' | ||
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* '''Structure:''' | * '''Structure:''' | ||
+ | ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4GYO 4GYO] (complex [[RapJ]]-[[PhrC]]) {{PubMed|23526881}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34327 O34327] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O34327 O34327] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rapJ_304430_305551_1 rapJ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rapJ_304430_305551_1 rapJ] {{PubMed|22383849}} | ||
− | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' |
Revision as of 17:41, 27 March 2013
- Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P
Gene name | rapJ |
Synonyms | ycdE |
Essential | no |
Product | response regulator aspartate phosphatase |
Function | control of the phosphorelay |
Gene expression levels in SubtiExpress: rapJ | |
Interactions involving this protein in SubtInteract: RapJ | |
MW, pI | 44 kDa, 4.902 |
Gene length, protein length | 1119 bp, 373 aa |
Immediate neighbours | cwlK, ycdF |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
protein modification, phosphorelay, transcription factors and their control
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU02820
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Protein family: RAP family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide PhrC, the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. PubMed
- Modification:
- Cofactor(s):
Database entries
- UniProt: O34327
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: rapJ (according to DBTBS)
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References