Difference between revisions of "RapJ"

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* '''Domains:'''  
 
* '''Domains:'''  
 
+
** RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide [[PhrC]], the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. {{PubMed|23526881}}
 
* '''Modification:'''
 
* '''Modification:'''
  
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* '''Structure:'''
 
* '''Structure:'''
 +
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4GYO 4GYO] (complex [[RapJ]]-[[PhrC]]) {{PubMed|23526881}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34327 O34327]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34327 O34327]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rapJ_304430_305551_1 rapJ] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rapJ_304430_305551_1 rapJ] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  

Revision as of 17:41, 27 March 2013

Gene name rapJ
Synonyms ycdE
Essential no
Product response regulator aspartate phosphatase
Function control of the phosphorelay
Gene expression levels in SubtiExpress: rapJ
Interactions involving this protein in SubtInteract: RapJ
MW, pI 44 kDa, 4.902
Gene length, protein length 1119 bp, 373 aa
Immediate neighbours cwlK, ycdF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RapJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RapJ expression.png















Categories containing this gene/protein

protein modification, phosphorelay, transcription factors and their control

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU02820

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: RAP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • RapJ is made up of the C-terminal tetratricopeptide repeat (TPR) domain that is connected by a flexible helix containing linker to the N-terminal 3-helix bundle. Upon binding of the regulating peptide PhrC, the 3-helix bundle and the linker helix undergo a conformational change to form a TPR-like fold that merges with the existing C-terminal TPR domain. PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: rapJ (according to DBTBS)
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References