Difference between revisions of "MetI"

From SubtiWiki
Jump to: navigation, search
Line 124: Line 124:
  
 
* '''Mutant:'''
 
* '''Mutant:'''
 +
** 1A941 ( ''metI''::''spec''), {{PubMed|11832514}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A941&Search=1A941 BGSC]
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 12:21, 19 September 2012

  • Description: O-succinylhomoserine lyase (L-cysteine, H2S, methanethiol, elimination)

Gene name metI
Synonyms yjcI
Essential no
Product O-succinylhomoserine lyase
(L-cysteine, H2S, methanethiol, elimination)
Function biosynthesis of methionine
Gene expression levels in SubtiExpress: metI
Metabolic function and regulation of this protein in SubtiPathways:
Cys, Met & Sulfate assimilation
MW, pI 41 kDa, 5.019
Gene length, protein length 1119 bp, 373 aa
Immediate neighbours yjcH, metC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YjcI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MetI expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

S-box

The gene

Basic information

  • Locus tag: BSU11870

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References