Difference between revisions of "SacB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of sucrose, production of levan  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of sucrose, production of levan  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU34450 sacB]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU34450 sacB]
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pbpE]]'', ''[[levB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pbpE]]'', ''[[levB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15450&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU34450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU34450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU34450 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:sacB_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:sacB_context.gif]]

Revision as of 13:55, 13 May 2013

  • Description: levansucrase

Gene name sacB
Synonyms
Essential no
Product levansucrase
Function utilization of sucrose, production of levan
Gene expression levels in SubtiExpress: sacB
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism, Stress
MW, pI 52 kDa, 6.175
Gene length, protein length 1419 bp, 473 aa
Immediate neighbours pbpE, levB
Sequences Protein DNA Advanced_DNA
Genetic context
SacB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SacB expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

DegU regulon, SacY regulon

The gene

Basic information

  • Locus tag: BSU34450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1PT2 (complex with sucrose), 1OYG
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by sucrose (at high concentration) (SacY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Massimiliano Marvasi, Pieter T Visscher, Lilliam Casillas Martinez
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS Microbiol Lett: 2010, 313(1);1-9
[PubMed:20735481] [WorldCat.org] [DOI] (I p)

Original publications