Difference between revisions of "Tpi"

From SubtiWiki
Jump to: navigation, search
Line 8: Line 8:
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''tpiA''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''tpiA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || yes
+
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || triosephosphate isomerase
 
|style="background:#ABCDEF;" align="center"| '''Product''' || triosephosphate isomerase
Line 39: Line 39:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
+
<br/><br/>
<br/><br/><br/><br/>
 
 
 
 
 
<br/><br/><br/><br/><br/><br/>
 
  
  
Line 60: Line 56:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
+
* essential {{PubMed|12682299}}, non-essential according to {{PubMed|23420519}}   
* Essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
Line 121: Line 116:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tpiA_3479405_3480166_-1 tpi] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tpiA_3479405_3480166_-1 tpi] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|11489127}}  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11489127}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 155: Line 150:
 
=References=
 
=References=
  
<pubmed>12850135 11489127 17505547 8021172 17218307 8580851 19193632 21821766</pubmed>
+
<pubmed>12850135 11489127 17505547 8021172 17218307 8580851 19193632 21821766 23420519</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:35, 14 April 2013

  • Description: triose phosphate isomerase, glycolytic/ gluconeogenic enzyme

Gene name tpi
Synonyms tpiA
Essential no
Product triosephosphate isomerase
Function enzyme in glycolysis/ gluconeogenesis
Gene expression levels in SubtiExpress: tpi
Interactions involving this protein in SubtInteract: Tpi
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism, Sugar catabolism
MW, pI 26,9 kDa, 4.79
Gene length, protein length 759 bp, 253 amino acids
Immediate neighbours pgm, pgk
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Tpi context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Tpi expression.png
















Categories containing this gene/protein

carbon core metabolism, phosphoproteins

This gene is a member of the following regulons

CggR regulon

The gene

Basic information

  • Locus tag: BSU33920

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate (according to Swiss-Prot)
  • Protein family: triosephosphate isomerase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-213 PubMed
  • Cofactor(s):
  • Effectors of protein activity: inhibited by 2-phosphoglycolate (in B. stearothermophilus) PubMed

Database entries

  • Structure: 1BTM (complex with 2-phosphoglycolic acid, Geobacillus stearothermophilus), complex with 2-phosphpoglycolic acid, Geobacillus stearothermophilus NCBI
  • KEGG entry: [3]

Additional information

  • extensive information on the structure and enzymatic properties of Tpi can be found at Proteopedia

Expression and regulation

  • Regulatory mechanism: transcription repression by CggR PubMed
  • Additional information:

Biological materials

  • Mutant: GP700 (cat), available in Stülke lab
  • Expression vector:
    • pGP394 (N-terminal His-tag, in pWH844), available in Stülke lab
    • pGP89 (N-terminal Strep-tag, for SPINE, expression in B. subtilis), available in Stülke lab
    • pGP1511 (expression in B. subtilis, in pBQ200), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References