Difference between revisions of "YmcB"

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|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA maturation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || tRNA maturation
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU17010 ymcB]
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 6.873   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 57 kDa, 6.873   

Revision as of 09:57, 7 August 2012

  • Description: tRNA methylthiotransferase

Gene name ymcB
Synonyms
Essential no
Product tRNA methylthiotransferase
Function tRNA maturation
Gene expression levels in SubtiExpress: ymcB
MW, pI 57 kDa, 6.873
Gene length, protein length 1527 bp, 509 aa
Immediate neighbours kbl, ymcA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YmcB context.png
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YmcB expression.png
























Categories containing this gene/protein

translation

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU17010

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: modifies N(6)-isopentenyladenosine (i(6)A) to 2-methylthio-N(6)-isopentenyladenosine (ms(2)i(6)A) in tRNA PubMed
  • Protein family: MiaB subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YqeV

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1109 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Brian P Anton, Susan P Russell, Jason Vertrees, Simon Kasif, Elisabeth A Raleigh, Patrick A Limbach, Richard J Roberts
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.
Nucleic Acids Res: 2010, 38(18);6195-205
[PubMed:20472640] [WorldCat.org] [DOI] (I p)