Difference between revisions of "LcfA"

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* '''Locus tag:''' BSU28560
 
* '''Locus tag:''' BSU28560
 
[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=lcfA_2918646_2920328_-1 Expression]
 
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 10:29, 26 January 2012

  • Description: long chain acyl-CoA synthetase, involved in surfactin production

Gene name lcfA
Synonyms
Essential no
Product long chain acyl-CoA synthetase
Function fatty acid degradation
Metabolic function and regulation of this protein in SubtiPathways:
Fatty acid degradation
MW, pI 62 kDa, 6.119
Gene length, protein length 1680 bp, 560 aa
Immediate neighbours fadR, yshE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LcfA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

  • Locus tag: BSU28560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed
  • Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed