Difference between revisions of "CysE"

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(Extended information on the protein)
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* '''Locus tag:''' BSU00930
 
* '''Locus tag:''' BSU00930
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[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cysE_112800_113453_1 Expression]
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===

Revision as of 12:09, 24 January 2012

  • Description: serine O-acetyltransferase

Gene name cysE
Synonyms cysA
Essential no
Product serine O-acetyltransferase
Function biosynthesis of cysteine
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging, Cys, Met & Sulfate assimilation
MW, pI 23 kDa, 9.489
Gene length, protein length 651 bp, 217 aa
Immediate neighbours gltX, cysS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
CysE context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU00930

Expression

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine (according to Swiss-Prot)
  • Protein family: transferase hexapeptide repeat family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1T3D (from Escherichia coli, 48% identity, 64% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Valerie E Pye, Andrew P Tingey, Robert L Robson, Peter C E Moody
The structure and mechanism of serine acetyltransferase from Escherichia coli.
J Biol Chem: 2004, 279(39);40729-36
[PubMed:15231846] [WorldCat.org] [DOI] (P p)

Y Gagnon, R Breton, H Putzer, M Pelchat, M Grunberg-Manago, J Lapointe
Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis.
J Biol Chem: 1994, 269(10);7473-82
[PubMed:7510287] [WorldCat.org] (P p)