Difference between revisions of "PGP1460"
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The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the [[SPINE]] method. Detailed information about the multiple cloning site can be found here: [[pGP382]]. Gene cloned into this vector require a Shine-Dalgarno sequence. | The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and kanamycin in ''E. coli'' and ''B. subtilis'', respectively. To transform ''B. subtilis'', the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the [[SPINE]] method. Detailed information about the multiple cloning site can be found here: [[pGP382]]. Gene cloned into this vector require a Shine-Dalgarno sequence. | ||
Revision as of 13:25, 28 September 2010
File:PGP1460.jpeg
pGP1460: click to enlarge
The vector was constructed in the Stülke lab and it is suitable for constitutive expression of C-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI. Just like pGP382 it can be used for the SPINE method. Detailed information about the multiple cloning site can be found here: pGP382. Gene cloned into this vector require a Shine-Dalgarno sequence.
Sequencing primers:
- M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
- M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
