Difference between revisions of "Methods"
| Line 3: | Line 3: | ||
*Bacterial Two Hybrid: [[BACTH]] | *Bacterial Two Hybrid: [[BACTH]] | ||
| − | *[[SPINE]]: A method to detect ''in vivo'' protein-protein interactions | + | *[[SPINE]]: A method to detect ''in vivo'' [[protein-protein interactions]] |
* introduction of markerfree deletions: {{PubMed|20057169}} | * introduction of markerfree deletions: {{PubMed|20057169}} | ||
| Line 15: | Line 15: | ||
* Standard operation procedures ([[SOP]]s) of the SYSMO-BACELL consortium | * Standard operation procedures ([[SOP]]s) of the SYSMO-BACELL consortium | ||
| − | * ''in vivo'' detection of protein-protein interaction using [[DivIVA]] and GFP {{PubMed|19698693}} | + | * ''in vivo'' detection of [[protein-protein interaction]] using [[DivIVA]] and GFP {{PubMed|19698693}} |
* ChAP-chip: A modified ChIP-chip protocol for the ''in vivo'' identification of binding sites of DNA-binding proteins {{PubMed|17932079}} | * ChAP-chip: A modified ChIP-chip protocol for the ''in vivo'' identification of binding sites of DNA-binding proteins {{PubMed|17932079}} | ||
Revision as of 21:32, 3 January 2011
Here you can find a list of methods for working with Bacillus subtilis:
- Bacterial Two Hybrid: BACTH
- SPINE: A method to detect in vivo protein-protein interactions
- introduction of markerfree deletions: PubMed
- generation of markerfree mutations: PubMed
- Mechanical cell disruption and extraction of RNA from B. subtilis: Media:SOP-RNA.pdf
- transformation of wild-type B. subtilis strains incl. NCIB3610: PubMed
- Standard operation procedures (SOPs) of the SYSMO-BACELL consortium
- in vivo detection of protein-protein interaction using DivIVA and GFP PubMed
- ChAP-chip: A modified ChIP-chip protocol for the in vivo identification of binding sites of DNA-binding proteins PubMed
