Difference between revisions of "GlmM"

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**pGP1400: (expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
 
**pGP1400: (expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
 +
 +
**pGP1402: (cloning vector for glmM (GlmM S100A) in ''E. coli'', in [[pBlueskript KS]]), available in [[Stülke]] lab
 +
 +
**pGP1403: (expression of GlmM (S100A) in ''B. subtilis'' in [[pBQ200]]), available in [[Stülke]] lab
 +
 +
**pGP1405: (expression, purification of GlmM (S100A) in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Stülke]] lab
 
   
 
   
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 21:19, 18 March 2010

  • Description: phosphoglucosamine mutase, required for cell wall synthesis

Gene name glmM
Synonyms ybbT
Essential yes PubMed
Product phosphoglucosamine mutase, required for cell wall synthesis
Function cell wall synthesis
MW, pI 48 kDa, 4.677
Gene length, protein length 1344 bp, 448 aa
Immediate neighbours ybbR, glmS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbbT context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU01770

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate (according to Swiss-Prot)
  • Protein family: phosphohexose mutase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-100 PubMed, this is an autophosphorylation PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • pGP400: (expression in B. subtilis in pBQ200), available in Stülke lab
    • pGP1400: (expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Stülke lab
    • pGP1403: (expression of GlmM (S100A) in B. subtilis in pBQ200), available in Stülke lab
    • pGP1405: (expression, purification of GlmM (S100A) in E. coli with N-terminal His-tag, in pWH844), available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References