aimA

168

general amino acid importer, low affinity symporter for [metabolite|glutamate]/H⁺, [metabolite|serine]/H⁺, [metabolite|asparagine]/H⁺, [metabolite|glycine][metabolite|]/H⁺, [metabolite|diaminopropionic acid]/H⁺, [metabolite|alanine]/H⁺, and [metabolite|beta-alanine]/H⁺

locus: BSU_02120

Molecular weight: 59.03 kDa

pI: 9.82

Protein length: 539 aa

Gene length: 1620 bp

function: low affinity uptake of [metabolite|serine], [metabolite|glutamate], [metabolite|asparagine], [metabolite|glycine], [metabolite|alanine], and [metabolite|beta-alanine]

product: amino acid importer A

essential: no

synonyms: aimA, ybeC

List of homologs in different organisms, belongs to COG0531 (Galperin et al., 2021)

Gene

Coordinates: 231,348  232,967

Phenotypes of a mutant

• resistant to [metabolite|serine] [pubmed|32743959]
• inactivation of [gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA] facilitates the adaptation a strain lacking [metabolite|c-di-AMP] to growth on medium containing [metabolite|glutamate] [pubmed|33481774]
• a [gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA] [gene|9AD0E43119BDC65D5F67CE55CD9417E1E6BAE058|aimB] [gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP] triple mutant is resistant to [metabolite|diaminopropionic acid] [pubmed|38470260]
• a [gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA] [gene|219586A3F378DC38EA076FD39B99D41136BDE722|alaP] double mutant is resistant to L-[metabolite|alanine] [pubmed|38470260]
• a [gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA] [gene|219586A3F378DC38EA076FD39B99D41136BDE722|alaP] double mutant is resistant to [metabolite|beta-alanine] [pubmed|38470260]
• a [gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA] mutant is resistant to [metabolite|glycine] [pubmed|38470260]

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The protein

Catalyzed reaction/ biological activity

• uptake of [metabolite|serine] [pubmed|32743959], [metabolite|glutamate] [pubmed|33481774], [metabolite|asparagine] [pubmed|38193659], [metabolite|glycine] [pubmed|38470260], [metabolite|diaminopropionic acid] [pubmed|38470260], [metabolite|alanine] [pubmed|38470260], and [metabolite|beta-alanine] [pubmed|38470260] (symport with H⁺)

Protein family

• [wiki|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

Structure

• [PDB|6F2G] from Carnobacterium sp. AT7, 24.8% identity [pubmed|31000719]
• [AF|Q45577]

Kinetic information

• K_S for [metabolite|glutamate]:
• - 3.2 mM (at external KCl concentration of 0.1 mM) [pubmed|33481774]
• - 2.3 mM (at external KCl concentration of 5 mM) [pubmed|33481774]

Paralogous protein(s)

• [protein|3AC4ED647836A1633DC3439BB4F3A74FA78CF2DB|yveA]

[wiki|Localization]

• membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]

  description: [pubmed|22383849]

Biological materials

Mutant

• GP3590 (Δ[gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::''phleo''), available in [wiki|Jörg Stülke]'s lab [pubmed|38193659]
• GP1886 Δ[gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::cat, available in [wiki|Jörg Stülke]'s lab [pubmed|32743959]
• GP2786 Δ[gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::aphA3, available in [wiki|Jörg Stülke]'s lab [pubmed|32743959]
• MGNA-B963 (ybeC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1962 NBRP B. subtilis, Japan]
• BKE02120 ([gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCGTAAACACCTTTCA,  downstream forward: _UP4_TAAAATATAGAAGAAAACCT
• BKK02120 ([gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATCGTAAACACCTTTCA,  downstream forward: _UP4_TAAAATATAGAAGAAAACCT
• GP2831 (''Δ''''[gene|533EEB1A454D29800C9A00A0BE107ECEE2138DAB|gltT]''::''aphA3'' ''Δ''''[gene|151A61315ADAA7213502F15C0C0ED636A4C3EA7C|aimA]''::''phleo''), available in [wiki|Jörg Stülke]'s lab

FLAG-tag construct

• GP3062 ''ybeC-3xFLAG spc'' (based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab

lacZ fusion

• pGP2287 (in [wiki|pAC5]) (GP2965), available in [wiki|Jörg Stülke]'s lab [pubmed|32743959]

References

• 18763711,31000719,32743959,33481774,38193659,38470260