Difference between revisions of "TagT"

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* '''Description:''' write here <br/><br/>
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* '''Description:''' phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' ||  
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|style="background:#ABCDEF;" align="center"| '''Product''' || phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35840 tagT]
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=TagT TagT]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 9.554   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 35 kDa, 9.554   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywtG]]'', ''[[ywtE]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywtG]]'', ''[[ywtE]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywtF_nucleotide.txt    Gene sequence     (+200bp)  ]'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35840 DNA_with_flanks]
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ywtF_protein.txt Protein sequence]'''
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|-
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
 
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywtF_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ywtF_context.gif]]
 +
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywtF_3694239_3695207_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:tagT_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35840]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/>
 +
 +
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[cell wall synthesis]]}},
 +
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
 +
{{SubtiWiki category|[[membrane proteins]]}}
  
−
<br/><br/>
+
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[SigM regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Basic information ===
 
=== Basic information ===
  
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* '''Coordinates:'''
+
* '''Locus tag:''' BSU35840
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* a ''[[tagT]]'' mutant has no phenoype, the triple ''[[tagT]] [[tagU]] [[tagV]]'' mutant is unable to grow under normal conditions {{PubMed|21964069}}
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35840&redirect=T BSU35840]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywtF.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ywtF.html]
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
−
* '''Protein family:'''
+
* '''Protein family:''' LytR-Cps2a-Psr family  {{PubMed|19099556}}
  
−
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[TagU]], [[TagV]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Modification:'''
 
* '''Modification:'''
  
−
* '''Cofactor(s):'''
+
* '''Cofactor(s):''' Mg(2+) {{PubMed|21964069}}
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
−
* '''Interactions:'''
+
* '''[[SubtInteract|Interactions]]:'''  
 +
** [[MreB]]-[[TagT]] {{PubMed|21964069}}
  
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* '''Localization:'''
+
* '''[[Localization]]:'''
 +
** cytoplasmic membrane (with extracytoplasmic catalaytic domain) {{PubMed|21964069}}
 +
** extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35840&redirect=T BSU35840]
  
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* '''Structure:'''
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* '''Structure:'''  
 +
** [http://www.pdb.org/pdb/explore/explore.do?structureId=3MEJ 3MEJ]
 +
** [http://www.rcsb.org/pdb/explore/explore.do?structureId=4DE9 4DE9] {{PubMed|22432711}}
  
−
* '''Swiss prot entry:'''
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/Q7WY78 Q7WY78]
  
−
* '''KEGG entry:'''
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35840]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  
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* '''Operon:''' ''ywtF'' {{PubMed|9353933}}
 +
 
 +
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ywtF_3694239_3695207_1 tagT] {{PubMed|22383849}}
  
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* '''Sigma factor:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
+
* '''[[Sigma factor]]:''' [[SigM]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** expressed at high salt concentrations ([[SigM]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18179421 Pubmed]
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 105 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 258 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
 +
==Reviews==
 +
<pubmed> 24024634 </pubmed>
 +
==Original publications==
 +
<pubmed>18957862, 9353933 18179421 19099556 21964069 22432711</pubmed>
  
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
+
[[Category:Protein-coding genes]]

Latest revision as of 11:45, 19 June 2014

  • Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

Gene name ywtF
Synonyms
Essential no
Product phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagT
Interactions involving this protein in SubtInteract: TagT
MW, pI 35 kDa, 9.554
Gene length, protein length 966 bp, 322 aa
Immediate neighbours ywtG, ywtE
Sequences Protein DNA DNA_with_flanks
Genetic context
YwtF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TagT expression.png















Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU35840

Phenotypes of a mutant

  • a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: LytR-Cps2a-Psr family PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed
    • extracellular (signal peptide) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at high salt concentrations (SigM) Pubmed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 105 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 258 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Stephanie Brown, John P Santa Maria, Suzanne Walker
Wall teichoic acids of gram-positive bacteria.
Annu Rev Microbiol: 2013, 67;313-36
[PubMed:24024634] [WorldCat.org] [DOI] (I p)

Original publications

Alice Eberhardt, Christopher N Hoyland, Daniela Vollmer, Stephanie Bisle, Robert M Cleverley, Ola Johnsborg, Leiv S Håvarstein, Richard J Lewis, Waldemar Vollmer
Attachment of capsular polysaccharide to the cell wall in Streptococcus pneumoniae.
Microb Drug Resist: 2012, 18(3);240-55
[PubMed:22432711] [WorldCat.org] [DOI] (I p)

Yoshikazu Kawai, Jon Marles-Wright, Robert M Cleverley, Robyn Emmins, Shu Ishikawa, Masayoshi Kuwano, Nadja Heinz, Nhat Khai Bui, Christopher N Hoyland, Naotake Ogasawara, Richard J Lewis, Waldemar Vollmer, Richard A Daniel, Jeff Errington
A widespread family of bacterial cell wall assembly proteins.
EMBO J: 2011, 30(24);4931-41
[PubMed:21964069] [WorldCat.org] [DOI] (I e)

Judith Hübscher, Lucas Lüthy, Brigitte Berger-Bächi, Patricia Stutzmann Meier
Phylogenetic distribution and membrane topology of the LytR-CpsA-Psr protein family.
BMC Genomics: 2008, 9;617
[PubMed:19099556] [WorldCat.org] [DOI] (I e)

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)

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