Difference between revisions of "Rny"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' essential!!!!, 4043 (''rny'' under p-spac control), GP193 (''rny'' under p-xyl control), available in [[Stülke]] lab
+
* '''Mutant:''' essential!!!!, 4043 (''rny'' under p-spac control, ''cat''), GP193 (''rny'' under p-xyl control, ''cat''), available in [[Stülke]] lab
  
 
* '''Expression vector:'''  
 
* '''Expression vector:'''  

Revision as of 15:51, 12 June 2009

  • Description: RNase Y, endoribonuclease, required for the processing of the gapA operon mRNA

Gene name rny
Synonyms ymdA
Essential yes
Product RNase Y
Function required for the processing
of the gapA operon mRNA
Regulatory function of this protein in SubtiPathways:
Central C-metabolism
MW, pI 58,7 kDa, 5.39
Gene length, protein length 1560 bp, 520 amino acids
Immediate neighbours pbpX, ymdB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Rny context.gif
This image was kindly provided by SubtiList






The gene

Basic information

  • Locus tag: BSU16960

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 3'-phosphate (according to Swiss-Prot) required for the processing of the gapA operon mRNA PubMed
  • Protein family: 2',3' cyclic nucleotide phosphodiesterase family (according to Swiss-Prot) 2',3' cyclic nucleotide phosphodiesterase family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • transmembrane domain (4–24)
    • KH domain (210–273)
    • HD domain (336–429)
  • Modification:
  • Cofactor(s): metal-dependent
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot), Single-pass membrane protein PubMed PubMed

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

required for the processing of the gapA operon mRNA

Expression and regulation

  • Sigma factor:
  • Regulation: constitutive
  • Regulatory mechanism:
  • Additional information: there is a terminator between rny and ymdB, most transcripts terminate there

Biological materials

  • Mutant: essential!!!!, 4043 (rny under p-spac control, cat), GP193 (rny under p-xyl control, cat), available in Stülke lab
  • Expression vector:

N-terminal Strep-tag, expression in E. coli, in pGP172: pGP441, available in Stülke lab

N-terminal Strep-tag, for SPINE, expression in B. subtilis, in pGP380: pGP775 , available in Stülke lab

wild type rny, expression in B. subtilis, in pBQ200: pGP1201, available in Stülke lab

there is also a series of domain constructs present in pBQ200, all available in Stülke lab

  • lacZ fusion: pGP459 (in pAC7), available in Stülke lab
  • GFP fusion: B. subtilis 3569 (amyE:: (p-xyl rny-gfpmut1-spc)), available in Errington lab
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

Labs working on this gene/protein

Harald Putzer, IBPC Paris, France Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

Your additional remarks

References