Difference between revisions of "DivIVA"

From SubtiWiki
Jump to: navigation, search
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU15420
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 81: Line 81:
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P71021 P71021]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P71021 P71021]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15420 BSU15420]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15420]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 11:30, 3 June 2009

  • Description: cell-division initiation protein (septum placement)

Gene name divIVA
Synonyms ylmJ
Essential no
Product cell-division initiation protein
Function septum placement
MW, pI 19 kDa, 4.846
Gene length, protein length 492 bp, 164 aa
Immediate neighbours ylmH, ileS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
DivIVA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU15420

Phenotypes of a mutant

Deletion of divIVA leads to filamentation and polar divisions that in turn cause a minicell phenotype. A divIVA mutant has a severe sporulation defect.

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: DivIVA is required for polar localisation of MinCD via MinJ. It also recruits RacA to the distal pole of the prespore.
  • Protein family: gpsB family (according to Swiss-Prot)
  • Paralogous protein(s): GpsB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: The Mycobacterium DivIVA homologue Wag31 is phosphorylated at T73.
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: DivIVA forms a ring underneath the invaginating membrane at the site of cell division and is enriched at both cell poles.

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: one gene cistron
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: divIVA::tet available from the Hamoen Lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle upon Tyne, United Kingdom [4]

Imrich Barak, Slovak Academy of Science, Bratislava, Slovakia homepage

Your additional remarks

References