Difference between revisions of "PGP3223"
(Created page with "C-terminal Osmy fusion (from E.coli), without ATG or STOP; 6xHIS tag in between GOI and OsmY, TEV-cleavage site is in front of the His tag to remove the tag completely Enzyme...") |
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Revision as of 09:16, 26 June 2019
C-terminal Osmy fusion (from E.coli), without ATG or STOP; 6xHIS tag in between GOI and OsmY, TEV-cleavage site is in front of the His tag to remove the tag completely
Enzymes: NdeI for fwd and XmaI for rev
