nasC
168
nitrate reductase (catalytic subunit)
locus
BSU_03310
Molecular weight
78.39 kDa
pI
6.73
function
utilization of nitrate
product
nitrate reductase (catalytic subunit)
essential
no
synonyms
nasC, narB, nasBB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3383 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
358,303 → 360,435
The protein
Protein family
[wiki|prokaryotic molybdopterin-containing oxidoreductase family] (according to UniProt)
[wiki|Domains]
[wiki|4Fe-4S Mo/W bis-MGD-type domain] (aa 19-77) (according to UniProt)
[wiki|Cofactors]
MoCo [Pubmed|11289299]
Fe-S cluster [Pubmed|11289299]
Structure
[PDB|2IV2] (from E. coli, 32% identity) [pubmed|16830149]
[AF|P42434]
Expression and Regulation
Operons
genes
[gene|3F87227DC3371746B3823730B6A7024D348C151E|nasB]-[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]-[gene|FAAC0F819AEC8E055CCEE86D93C8DE5584DC4B23|nasD]-[gene|A8998F46F68155A5C8AE2A2FBB95CA5A1635BB2B|nasE]-[gene|159A6D0D45B5F0F055228357FD9A3BF8E5A999E5|nasF]
description
[Pubmed|7836289]
regulation
''[protein|search|nasD]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]
regulatory mechanism
[protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: activation, [PubMed|8799114,9765565], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|7836289], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE03310 (Δ[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE03310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA, downstream forward: _UP4_TAAATTTTTCATAAAATTTT
BKK03310 (Δ[gene|EC8BF9A3BA88FE2A78C6F78A888E0D1E25C47BC5|nasC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK03310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAGCAGTCGTTCAGTCAAAA, downstream forward: _UP4_TAAATTTTTCATAAAATTTT
References
Reviews
Original Publications
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Time of last update: 2024-06-07 06:24:57
Author of last update: Melvin.boenninger