Difference between revisions of "SigM"

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(References)
(Phenotypes of a mutant)
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* ''sigM'' mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts {{PubMed|10216858}}
 
* ''sigM'' mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts {{PubMed|10216858}}
 
* increased sensitivity towards beta-lactam antibiotics {{PubMed|22211522}}
 
* increased sensitivity towards beta-lactam antibiotics {{PubMed|22211522}}
 +
* a ''[[sigM]] [[murJ]]'' double mutant is not viable (due to the lack of both lipid II flippases) {{PubMed|25918422}}
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 13:00, 8 May 2015

Gene name sigM
Synonyms yhdM
Essential no
Product RNA polymerase ECF-type sigma factor SigM
Function responsible for intrinsic resistance against beta-lactam antibiotics
Gene expression levels in SubtiExpress: sigM
Interactions involving this protein in SubtInteract: SigM
MW, pI 19 kDa, 6.77
Gene length, protein length 489 bp, 163 aa
Immediate neighbours yhdL, yhdN
Sequences Protein DNA DNA_with_flanks
Genetic context
SigM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigM expression.png















Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics

This gene is a member of the following regulons

SigM regulon

The SigM regulon

The gene

Basic information

  • Locus tag: BSU09520

Phenotypes of a mutant

  • SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
  • sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed
  • increased sensitivity towards beta-lactam antibiotics PubMed
  • a sigM murJ double mutant is not viable (due to the lack of both lipid II flippases) PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family, ECF subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
    • shortage of available bactoprenol causes SigM activation PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
    • shortage of available bactoprenol causes SigM activation PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

Reviews

The SigM regulon

Other original publications