TagV

• Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid
  
  

Gene name	tagV
Synonyms	yvhJ
Essential	no
Product	phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function	transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagV
MW, pI	43 kDa, 9.139
Gene length, protein length	1173 bp, 391 aa
Immediate neighbours	yvyE, tagO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, membrane proteins

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU35520

Phenotypes of a mutant

• a tagV mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

• BsubCyc: BSU35520

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LytR-Cps2a-Psr family PubMed

• Paralogous protein(s): TagT, TagU

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Mg(2+) PubMed

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed

Database entries

• BsubCyc: BSU35520

• Structure: 3NXH

• UniProt: P96499

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yvyE-tagV PubMed

• Expression browser: tagV PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Stephanie Brown, John P Santa Maria, Suzanne Walker
Wall teichoic acids of gram-positive bacteria.
Annu Rev Microbiol: 2013, 67;313-36
[PubMed:24024634] [WorldCat.org] [DOI] (I p)

Original publications

Yoshikazu Kawai, Jon Marles-Wright, Robert M Cleverley, Robyn Emmins, Shu Ishikawa, Masayoshi Kuwano, Nadja Heinz, Nhat Khai Bui, Christopher N Hoyland, Naotake Ogasawara, Richard J Lewis, Waldemar Vollmer, Richard A Daniel, Jeff Errington
A widespread family of bacterial cell wall assembly proteins.
EMBO J: 2011, 30(24);4931-41
[PubMed:21964069] [WorldCat.org] [DOI] (I e)

Judith Hübscher, Lucas Lüthy, Brigitte Berger-Bächi, Patricia Stutzmann Meier
Phylogenetic distribution and membrane topology of the LytR-CpsA-Psr protein family.
BMC Genomics: 2008, 9;617
[PubMed:19099556] [WorldCat.org] [DOI] (I e)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)