yorK

168

single-strand DNA-specific exonuclease

Locus: BSU_20350

Molecular weight: 65.60 kDa

Isoelectric point: 6.56

Protein length: 576 aa

Gene length: 1731 bp

Function: unknown

Product: single-strand DNA-specific exonuclease

Essential: no

Synonyms: yorK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0608 (Galperin et al., 2021)

Gene

Coordinates: 2,179,526 → 2,181,256

The protein

Protein family

• RecJ family (with RecJ, according to UniProt)

Domains

• DHH-DHHA1 domain

Structure

• 5F54 (PDB) (from Deinococcus radiodurans, the DHH-DHHA1 domain, 23% identity) PubMed
• O31903 (AlphaFold)

Modification

• phosphorylation on Tyr-473 PubMed

Paralogous protein(s)

• RecJ

Biological materials

Mutant

• BKE20350 (ΔyorK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCGCCAATTAGTCTATACT,  downstream forward: _UP4_CTTGTGTTTTAAGGGGGAGA
• BKK20350 (ΔyorK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCGCCAATTAGTCTATACT,  downstream forward: _UP4_CTTGTGTTTTAAGGGGGAGA

References

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