Difference between revisions of "IspE"
| Line 55: | Line 55: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00460&redirect=T BSU00460] | ||
* '''DBTBS entry:''' no entry | * '''DBTBS entry:''' no entry | ||
| Line 92: | Line 93: | ||
=== Database entries === | === Database entries === | ||
| + | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU00460&redirect=T BSU00460] | ||
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1uek 1UEK] (from ''E. coli'', 38% identity) {{PubMed|12771135}} | * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1uek 1UEK] (from ''E. coli'', 38% identity) {{PubMed|12771135}} | ||
Revision as of 12:46, 2 April 2014
- Description: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis
| Gene name | ispE |
| Synonyms | yabH , ipk |
| Essential | yes PubMed |
| Product | 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase |
| Function | MEP pathway of isoprenoid biosynthesis |
| Gene expression levels in SubtiExpress: ispE | |
| MW, pI | 31 kDa, 6.349 |
| Gene length, protein length | 867 bp, 289 aa |
| Immediate neighbours | sspF, purR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of lipids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00460
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU00460
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol (according to Swiss-Prot)
- Protein family: IspE subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- BsubCyc: BSU00460
- UniProt: P37550
- KEGG entry: [2]
- E.C. number: 2.7.1.148
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Becky M Hess, Junfeng Xue, Lye Meng Markillie, Ronald C Taylor, H Steven Wiley, Birgitte K Ahring, Bryan Linggi
Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics.
PLoS One: 2013, 8(6);e66104
[PubMed:23840410]
[WorldCat.org]
[DOI]
(I e)
Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547]
[WorldCat.org]
[DOI]
(P p)
Takashi Wada, Tomohisa Kuzuyama, Shinya Satoh, Seiki Kuramitsu, Shigeyuki Yokoyama, Satoru Unzai, Jeremy R H Tame, Sam-Yong Park
Crystal structure of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, an enzyme in the non-mevalonate pathway of isoprenoid synthesis.
J Biol Chem: 2003, 278(32);30022-7
[PubMed:12771135]
[WorldCat.org]
[DOI]
(P p)
