Difference between revisions of "GcaD"

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(References)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 +
**pGP2596: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 09:24, 14 March 2014

  • Description: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Gene name gcaD
Synonyms tms, tms26
Essential yes PubMed
Product bifunctional N-acetylglucosamine-1-phosphate

uridyltransferase/glucosamine-1-phosphate acetyltransferase

Function cell wall metabolism
Gene expression levels in SubtiExpress: gcaD
Metabolic function and regulation of this protein in SubtiPathways:
gcaD
MW, pI 49 kDa, 5.65
Gene length, protein length 1368 bp, 456 aa
Immediate neighbours spoVG, prs
Sequences Protein DNA DNA_with_flanks
Genetic context
GcaD context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
GcaD expression.png




























Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU00500

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1HM9 (from Streptococcus pneumoniae, 49% identity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • pGP2596: (IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References