Difference between revisions of "MgsA"

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=== Additional information===
 
=== Additional information===
 
  
 
=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Mutant:'''
 
* '''Mutant:'''
** GP67 (''mgsA''::''tet''), available in [[Stülke]] lab
+
** GP67 (''mgsA''::''tet'') {{PubMed|21992469}}, available in [[Jörg Stülke]]'s lab
** GP84 (''mgsA''::''pX2''(''cat'')), available in [[Stülke]] lab
+
** GP84 (''mgsA''::''pX2''(''cat'')), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
** pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Stülke]] lab
+
** pGP1301 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab
** pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
+
** pGP1180 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Jörg Stülke]]'s lab
** pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Stülke]] lab
+
** pGP1181 (C-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP382]]), available in [[Jörg Stülke]]'s lab
** pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP1459]]), available in [[Stülke]] lab   
+
** pGP2207 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP1459]]), available in [[Jörg Stülke]]'s lab   
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
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* '''GFP fusion:'''
 
* '''GFP fusion:'''
  
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Boris Görke| Görke]] lab
+
* '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab {{PubMed|21992469}}
  
* '''FLAG-tag construct:''' GP86 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
+
* '''FLAG-tag construct:''' GP86 (spc, based on [[pGP1331]]) {{PubMed|21992469}}, available in [[Jörg Stülke]]'s lab
  
 
* '''Antibody:'''
 
* '''Antibody:'''
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
[[Boris Görke]], University of Göttingen, Germany
+
* [[Boris Görke]], University of Vienna, Austria
[http://wwwuser.gwdg.de/~genmibio/goerke.html Homepage]
+
* [[JörgStülke]], University of Göttingen, Germany, [http://genmibio.uni-goettingen.de/index.php?id=90 Homepage]
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 17:15, 16 January 2014

  • Description: methylglyoxal synthase

Gene name mgsA
Synonyms ypjF, jojF
Essential no
Product methylglyoxal synthase
Function bypass of glycolysis
Gene expression levels in SubtiExpress: mgsA
Interactions involving this protein in SubtInteract: MgsA
Metabolic function and regulation of this protein in SubtiPathways:
mgsA
MW, pI 14 kDa, 4.919
Gene length, protein length 411 bp, 137 aa
Immediate neighbours bshB1, dapB
Sequences Protein DNA DNA_with_flanks
Genetic context
MgsA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MgsA expression.png















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

Spx regulon


The gene

Basic information

  • Locus tag: BSU22480

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Glycerone phosphate = methylglyoxal + phosphate (according to Swiss-Prot)
  • Protein family: RNase Z family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
    • non-phosphorylated Crh interacts with MgsA to inhibit its activity PubMed


Database entries

  • Structure: 1B93 (from Escherichia coli, 50% identity, 73% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced by diamide stess (thiol depletion) (Spx) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab PubMed
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ahmed Gaballa, Haike Antelmann, Chris J Hamilton, John D Helmann
Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx.
Microbiology (Reading): 2013, 159(Pt 10);2025-2035
[PubMed:23894131] [WorldCat.org] [DOI] (I p)

Rachit Jain, Yajun Yan
Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli.
Microb Cell Fact: 2011, 10;97
[PubMed:22074179] [WorldCat.org] [DOI] (I e)

Jens J Landmann, Ricarda A Busse, Jan-Hendrik Latz, Kalpana D Singh, Jörg Stülke, Boris Görke
Crh, the paralogue of the phosphocarrier protein HPr, controls the methylglyoxal bypass of glycolysis in Bacillus subtilis.
Mol Microbiol: 2011, 82(3);770-87
[PubMed:21992469] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)

D Saadat, D H Harrison
The crystal structure of methylglyoxal synthase from Escherichia coli.
Structure: 1999, 7(3);309-17
[PubMed:10368300] [WorldCat.org] [DOI] (P p)