Difference between revisions of "MutY"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08630 mutY]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU08630 mutY]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=mutY mutY]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.044   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 41 kDa, 6.044   

Revision as of 10:22, 7 January 2014

  • Description: A/G-specific adenine glycosylase, error prevention oxidized guanine system, releases adenines from 8-oxo-G:A mismatches

Gene name mutY
Synonyms yfhQ
Essential no
Product A/G-specific adenine glycosylase
Function DNA repair
Gene expression levels in SubtiExpress: mutY
Metabolic function and regulation of this protein in SubtiPathways:
mutY
MW, pI 41 kDa, 6.044
Gene length, protein length 1107 bp, 369 aa
Immediate neighbours yfhP, yfhS
Sequences Protein DNA DNA_with_flanks
Genetic context
YfhQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MutY expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

YfhP regulon

The gene

Basic information

  • Locus tag: BSU08630

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: releases adenines from 8-oxo-G:A mismatches
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1RRS (complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expression is constitutive throughout growth PubMed
    • expression is fivefold increased in a mutS-mutL mutant PubMed
    • YfhP may act as a negative transcription regulator of the mutY-fabL-sspE operon during exponential growth PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Bernardo N Debora, Luz E Vidales, Rosario Ramírez, Mariana Ramírez, Eduardo A Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Mismatch repair modulation of MutY activity drives Bacillus subtilis stationary-phase mutagenesis.
J Bacteriol: 2011, 193(1);236-45
[PubMed:20971907] [WorldCat.org] [DOI] (I p)

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)

Hiroki Yamamoto, Masao Mori, Junichi Sekiguchi
Transcription of genes near the sspE locus of the Bacillus subtilis genome.
Microbiology (Reading): 1999, 145 ( Pt 8);2171-2180
[PubMed:10463184] [WorldCat.org] [DOI] (P p)