Difference between revisions of "RbgA"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
* essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
* essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
* depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes
+
* depletion confers a slow growth phenotype, and induces an abnormality of the ''in vivo'' ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes {{PubMed|24335279,23700310}}
* premature 45S particles accumulated in YlqF-depleted cells lack several proteins, including [[rplP|L16]], [[rpmB|L28]], [[rpmGA|L33]], [[rpmI|L35]] and [[rpmJ|L36]] {{PubMed|23700310}}
+
* premature 45S particles accumulated in YlqF-depleted cells lack several proteins, including [[rplP|L16]], [[rpmB|L28]], [[rpmGA|L33]], [[rpmI|L35]] and [[rpmJ|L36]] {{PubMed|24335279,23700310}}
  
 
=== Database entries ===
 
=== Database entries ===
Line 79: Line 79:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
 
** RNA-binding domain at the C-terminus {{PubMed|23325847}}
 
** RNA-binding domain at the C-terminus {{PubMed|23325847}}
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** GTPase activity requires binding of [[rplP|L16]] to the 50S subunit of the [[ribosome]] {{PubMed|24335279}}
  
 
* '''[[SubtInteract|Interactions]]:'''  
 
* '''[[SubtInteract|Interactions]]:'''  
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==Original publications==
 
==Original publications==
<pubmed>12427945, 16390447 16997968 23325847 22267738 23700310 </pubmed>
+
<pubmed>12427945, 16390447 16997968 23325847 22267738 23700310 24335279</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:16, 18 December 2013

  • Description: assembly of the 50S subunit of the ribosome

Gene name rbgA
Synonyms ylqF
Essential yes PubMed
Product GTPase
Function ribosome assembly
Gene expression levels in SubtiExpress: rbgA
MW, pI 31 kDa, 9.528
Gene length, protein length 846 bp, 282 aa
Immediate neighbours rplS, rnhB
Sequences Protein DNA DNA_with_flanks
Genetic context
YlqF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RbgA expression.png















Categories containing this gene/protein

translation, essential genes, GTP-binding proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU16050

Phenotypes of a mutant

  • essential PubMed
  • depletion confers a slow growth phenotype, and induces an abnormality of the in vivo ribosome profile, with an increased level of 45S precursors and a decreased level of 70S ribosomes PubMed
  • premature 45S particles accumulated in YlqF-depleted cells lack several proteins, including L16, L28, L33, L35 and L36 PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: MTG1 subfamily (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
    • RNA-binding domain at the C-terminus PubMed
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews


Original publications