Difference between revisions of "PcrA"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pcrB]]'', ''[[ligA]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pcrB]]'', ''[[ligA]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06610 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU06610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU06610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU06610 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:pcrA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:pcrA_context.gif]]

Revision as of 09:38, 14 May 2013

  • Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer

Gene name pcrA
Synonyms yerF
Essential yes PubMed
Product ATP-dependent DNA helicase
Function plasmid rolling-circle replication, conjugative transfer of ICEBs1
Gene expression levels in SubtiExpress: pcrA
Interactions involving this protein in SubtInteract: PcrA
MW, pI 83 kDa, 5.767
Gene length, protein length 2217 bp, 739 aa
Immediate neighbours pcrB, ligA
Sequences Protein DNA DNA_with_flanks
Genetic context
PcrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PcrA expression.png















Categories containing this gene/protein

DNA repair/ recombination, mobile genetic elements, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
    • dismantles RecA filaments PubMed
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s): YjcD

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):

Database entries

  • Structure: 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Elisabeth Grohmann
Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements?
Mol Microbiol: 2010, 75(2);261-3
[PubMed:19943906] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed

Jacob Thomas, Catherine A Lee, Alan D Grossman
A conserved helicase processivity factor is needed for conjugation and replication of an integrative and conjugative element.
PLoS Genet: 2013, 9(1);e1003198
[PubMed:23326247] [WorldCat.org] [DOI] (I p)

Jeehae Park, Sua Myong, Anita Niedziela-Majka, Kyung Suk Lee, Jin Yu, Timothy M Lohman, Taekjip Ha
PcrA helicase dismantles RecA filaments by reeling in DNA in uniform steps.
Cell: 2010, 142(4);544-55
[PubMed:20723756] [WorldCat.org] [DOI] (I p)

Catherine A Lee, Ana Babic, Alan D Grossman
Autonomous plasmid-like replication of a conjugative transposon.
Mol Microbiol: 2010, 75(2);268-79
[PubMed:19943900] [WorldCat.org] [DOI] (I p)

Ye Yang, Shuo-Xing Dou, Hua Ren, Peng-Ye Wang, Xing-Dong Zhang, Min Qian, Bing-Yi Pan, Xu Guang Xi
Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding.
Nucleic Acids Res: 2008, 36(6);1976-89
[PubMed:18276648] [WorldCat.org] [DOI] (I p)

Syam P Anand, Haocheng Zheng, Piero R Bianco, Sanford H Leuba, Saleem A Khan
DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchange.
J Bacteriol: 2007, 189(12);4502-9
[PubMed:17449621] [WorldCat.org] [DOI] (P p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

M-F Noirot-Gros, P Soultanas, D B Wigley, S D Ehrlich, P Noirot, M-A Petit
The beta-propeller protein YxaL increases the processivity of the PcrA helicase.
Mol Genet Genomics: 2002, 267(3);391-400
[PubMed:12073041] [WorldCat.org] [DOI] (P p)

P Soultanas, M S Dillingham, P Wiley, M R Webb, D B Wigley
Uncoupling DNA translocation and helicase activity in PcrA: direct evidence for an active mechanism.
EMBO J: 2000, 19(14);3799-810
[PubMed:10899133] [WorldCat.org] [DOI] (P p)

S S Velankar, P Soultanas, M S Dillingham, H S Subramanya, D B Wigley
Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.
Cell: 1999, 97(1);75-84
[PubMed:10199404] [WorldCat.org] [DOI] (P p)

M A Petit, E Dervyn, M Rose, K D Entian, S McGovern, S D Ehrlich, C Bruand
PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication.
Mol Microbiol: 1998, 29(1);261-73
[PubMed:9701819] [WorldCat.org] [DOI] (P p)