Difference between revisions of "ThiC"

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=== Additional information===
 
=== Additional information===
  
:* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
+
* subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}
 +
 
 
=Expression and regulation=
 
=Expression and regulation=
* '''Operon:'''  
+
* '''Operon:''' ''[[thiC]]'' {{PubMed|22383849}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thiC_955895_957667_1 thiC] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thiC_955895_957667_1 thiC] {{PubMed|22383849}}
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** [[Thi-box]]: [[RNA switch]] {{PubMed|12376536}}
 
** [[Thi-box]]: [[RNA switch]] {{PubMed|12376536}}
  
* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
+
* '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation {{PubMed|17981983}}
  
 
=Biological materials =
 
=Biological materials =
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==Original publications==
 
==Original publications==
<pubmed>16291685 9370266 20509597 12376536 22517742</pubmed>
+
<pubmed>16291685 9370266 20509597 12376536 22517742 22383849</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:47, 21 April 2012

  • Description: biosynthesis of the pyrimidine moiety of thiamine

Gene name thiC
Synonyms thiA
Essential no
Product unknown
Function biosynthesis of thiamine
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 65 kDa, 5.262
Gene length, protein length 1770 bp, 590 aa
Immediate neighbours ygaJ, ygaK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiC expression.png




























Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU08790

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: thiC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-556 PubMed
    • phosphorylated on Ser-565, Ser-586 and Tyr-589 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed by thiamine and 2-methyl-4-amino-5-hydroxymethylpyrimidine PubMed
    • repressed by thiamine (Thi-box) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)


Original publications