Difference between revisions of "LutB"

From SubtiWiki
Jump to: navigation, search
(Phenotypes of a mutant)
Line 39: Line 39:
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
  
no growth with lactate as the single carbon source
+
no growth with lactate as the single carbon source [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
Line 54: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' oxidation of lactate to pyruvate
+
* '''Catalyzed reaction/ biological activity:''' oxidation of lactate to pyruvate [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
  
 
* '''Protein family:'''
 
* '''Protein family:'''
Line 90: Line 90:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[lutA]]-[[lutB]]-[[lutC]]''
+
* '''Operon:''' ''[[lutA]]-[[lutB]]-[[lutC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  
  
* '''Regulation:''' induction by lactate
+
* '''Regulation:''' induction by lactate [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
  
* '''Regulatory mechanism:''' dual repression by [[LutR]] (inducer lactate) and [[SinR]]
+
* '''Regulatory mechanism:''' dual repression by [[LutR]] (inducer lactate) and [[SinR]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19201793 PubMed]
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  

Revision as of 15:45, 10 February 2009

  • Description: lactate catabolic enzyme

Gene name lutB
Synonyms yvfW
Essential
Product
Function lactate utilization
MW, pI 53 kDa, 5.848
Gene length, protein length 1437 bp, 479 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
YvfW context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

no growth with lactate as the single carbon source PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: oxidation of lactate to pyruvate PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation: induction by lactate PubMed
  • Regulatory mechanism: dual repression by LutR (inducer lactate) and SinR PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed