Difference between revisions of "PcrA"

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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PJR 2PJR] (�product complex�, complex with sulphate ion, Geobacillus stearothermophilus),  [http://www.rcsb.org/pdb/explore.do?structureId=1PJR 1PJR] (�substrate complex�, complex with DNA, Geobacillus stearothermophilus)
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PJR 2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus''),  [http://www.rcsb.org/pdb/explore.do?structureId=1PJR 1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34580 O34580]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34580 O34580]
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<pubmed> 19943906 </pubmed>
 
<pubmed> 19943906 </pubmed>
 
==Original Publications==
 
==Original Publications==
<pubmed>12073041,9701819 ,16267290, 19943900 20723756 </pubmed>
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<pubmed>12073041,9701819 ,16267290, 19943900 20723756 18276648 17449621 10899133 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:54, 15 October 2010

  • Description: ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer

Gene name pcrA
Synonyms yerF
Essential yes PubMed
Product ATP-dependent DNA helicase
Function plasmid rolling-circle replication, conjugative transfer of ICEBs1
MW, pI 83 kDa, 5.767
Gene length, protein length 2217 bp, 739 aa
Immediate neighbours pcrB, ligA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PcrA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU06610

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop PubMed
    • dismantles RecA filaments PubMed
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure: 2PJR (product complex, complex with sulphate ion, Geobacillus stearothermophilus), 1PJR (substrate complex, complex with DNA, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Elisabeth Grohmann
Autonomous plasmid-like replication of Bacillus ICEBs1: a general feature of integrative conjugative elements?
Mol Microbiol: 2010, 75(2);261-3
[PubMed:19943906] [WorldCat.org] [DOI] (I p)

Original Publications