Difference between revisions of "YvcL"

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(Your additional remarks)
(Database entries)
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3HYI 3HYI] (Crystal structure of full-length DUF199/WhiA from Thermatoga maritima), [http://www.rcsb.org/pdb/explore.do?structureId=3HYJ 3HYJ] (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3HYI 3HYI] (Crystal structure of full-length DUF199/WhiA from ''Thermatoga maritima''), [http://www.rcsb.org/pdb/explore.do?structureId=3HYJ 3HYJ] (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O06975 O06975]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O06975 O06975]

Revision as of 17:39, 27 September 2010

  • Description: WhiA family protein conserved and ubiquitous among gram-positive bacteria, putative transcription factor

Gene name yvcL
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 36 kDa, 9.479
Gene length, protein length 948 bp, 316 aa
Immediate neighbours crh, yvcK
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YvcL context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU34750

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: WhiA family PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains: suggested HTH domain PubMed
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 3HYI (Crystal structure of full-length DUF199/WhiA from Thermatoga maritima), 3HYJ (Crystal structure of the N-terminal LAGLIDADG domain of DUF199/WhiA)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: very weak stimuation of expression by citrate and succinate PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP231 (amyE::PrpsJ-lacZ, yvcL::cat) available in Stülke lab; plasmid pGP1468 was used to disrupt the gene.
  • Expression vector: pGP1469 for expression in B. subtilis (in pGP382, with C-terminal STREP-TAG)
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

WhiA orthologues are present in all Gram-positive bacteria and best characterized by the WhiA sporulation control factor in Streptomyces coelicolor. They are thought to act as genetic regulators that have evolved from LAGLIDADG homing endonucleases (LHE).

References