Difference between revisions of "LexA"

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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=1JHH 1JHH] (from ''E. coli'', S119A mutant, 33% identity, 52% similarity) {{PubMed|11551506}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P31080 P31080]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P31080 P31080]
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=References=
 
=References=
  
<pubmed>8899710, 9045831 9555905 8969214, 1657879,,16267290 11532142 </pubmed>
+
<pubmed>8899710, 9045831 9555905 8969214, 1657879,,16267290 11532142 11551506 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:34, 3 February 2010

  • Description: transcriptional repressor of the SOS regulon

Gene name lexA
Synonyms dinR
Essential no
Product transcriptional repressor of the SOS regulon
Function regulation of DNA damage repair
MW, pI 22 kDa, 5.155
Gene length, protein length 615 bp, 205 aa
Immediate neighbours fosB, yneA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LexA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU17850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Represses uvrB, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)
  • Protein family: peptidase S24 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 1JHH (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References