yxkO

168

ADP/ATP-dependent NAD(P)H-hydrate dehydratase, general stress protein, survival of ethanol stress

locus: BSU_38720

Molecular weight: 29.72 kDa

pI: 5.49

Protein length: 276 aa

Gene length: 831 bp

function: survival of ethanol stress, repair of hydrated NAD(P)H

product: ADP/ATP-dependent NAD(P)H-hydrate dehydratase

essential: no

synonyms: yxkO

List of homologs in different organisms, belongs to COG0063 (Galperin et al., 2021)

Gene

Coordinates: 3,972,448  3,973,278

Advertisement

The protein

Catalyzed reaction/ biological activity

• (6S)-NADHX + ADP --> AMP + H⁺ + NADH + phosphate (according to UniProt)
• (6S)-NADPHX + ADP --> AMP + H⁺ + NADPH + phosphate (according to UniProt)

Protein family

• NnrD/CARKD family (single member, according to UniProt)

[wiki|Domains]

• YjeF C-terminal domain (aa 7-275) (according to UniProt)

Structure

• [PDB|1KYH] [pubmed|12457846]
• [AF|P94368]

Expression and Regulation

Operons

• genes: [gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]

  description: [Pubmed|10746760]

  regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

Biological materials

Mutant

• MGNA-B749 (yxkO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1748 NBRP B. subtilis, Japan]
• BKE38720 ([gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC,  downstream forward: _UP4_TTTGAATGATAAAAAAGCTG
• BKK38720 ([gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38720 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC,  downstream forward: _UP4_TTTGAATGATAAAAAAGCTG

References

• 15805528,10746760,12457846,22940582,25393291,17702456