yxeM

168

putative S-(2-succino)cysteine [wiki|ABC transporter] (binding protein)

locus: BSU_39500

Molecular weight: 29.16 kDa

pI: 9.09

Protein length: 264 aa

Gene length: 795 bp

function: uptake and utilization of S-(2-succino)cysteine

product: putative S-(2-succino)cysteine [wiki|ABC transporter] (binding protein)

essential: no

synonyms: yxeM

List of homologs in different organisms, belongs to COG0834 (Galperin et al., 2021)

Gene

Coordinates: 4,059,488 → 4,060,282

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The protein

Protein family

• [wiki|bacterial solute-binding protein 3 family] (according to UniProt)

Structure

• [PDB|4ZV1] (synthetic, 38% identity) [pubmed|26853627]
• [AF|P54952]

[wiki|Localization]

• associated to the membrane (via [protein|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|19E6EF9CCA31440EAC046F8D6CA98B6A08589D58|yxeK]-[gene|EBC2B9107AD50DD8F8174FC0BE4AD90DEE6A7CCA|snaB]-[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]-[gene|FFD5F07480A0E5A74852C69C436EE289BA72FC7F|yxeN]-[gene|F8C800CF71D21A1F3265977C4F0EE191D0FFE36A|yxeO]-[gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB]-[gene|7DDB283173267AA106DC277787AAAB4F4F9975F4|yxeQ]

  description: [Pubmed|10746760]

  regulation

  • repressed in the presence of cysteine ([protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]) [Pubmed|16513748]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16513748], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the amount of the mRNA is substantially decreased upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• MGNA-B782 (yxeM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1781 NBRP B. subtilis, Japan]
• 1A950 ( ''yxeM''::''cat''), [Pubmed|15262924], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A950&Search=1A950 BGSC]
• BKE39500 (Δ[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGATTTTCCCGCCTTT,  downstream forward: _UP4_TAAGAAACGGCGGTGACTCA
• BKK39500 (Δ[gene|D96EEDABB73AC66E6AB772431819E7C767DC8B9A|yxeM]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39500 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTGATTTTCCCGCCTTT,  downstream forward: _UP4_TAAGAAACGGCGGTGACTCA

References

• 10092453,12193636,16513748,10746760,21815947,29626092,26853627