yxeH

168

[wiki|HAD superfamily] sugar phosphatase

locus: BSU_39550

Molecular weight: 30.07 kDa

pI: 4.64

Protein length: 270 aa

Gene length: 813 bp

function: dephosphorylation of phosphosugars

product: sugar phosphatase

essential: no

synonyms: yxeH

List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 4,063,684  4,064,496

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The protein

Catalyzed reaction/ biological activity

• dephosphorylation of alpha-glucose-1-P and erythrose-4-P [pubmed|16990279]

Protein family

• [wiki|HAD superfamily] (according to UniProt)
• [wiki|Cof family] (according to UniProt)

Structure

• [PDB|1RKQ] (YidA from E. coli, 51% identity)
• [AF|P54947]

Expression and Regulation

Operons

• genes: [gene|637445F04D3F96D83DE46070CD6958A27EEB45DD|yxeF]-[gene|1C19EAEA89E95440204186A2644DDE89CF9F597F|yxeG]-[gene|23868F99A31FC20E9CB5FB2B6DBCF182C63D2335|yxeH]

  description: [Pubmed|10746760]

Biological materials

Mutant

• MGNA-B707 (yxeH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1706 NBRP B. subtilis, Japan]
• BKE39550 ([gene|23868F99A31FC20E9CB5FB2B6DBCF182C63D2335|yxeH]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGCATCTCCTTCATG,  downstream forward: _UP4_TAATAAAAAATCCAGCCTTC
• BKK39550 ([gene|23868F99A31FC20E9CB5FB2B6DBCF182C63D2335|yxeH]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39550 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCTGCATCTCCTTCATG,  downstream forward: _UP4_TAATAAAAAATCCAGCCTTC

References

• 16672620,25527541,16990279