dltX

168

required for D-alanylation of lipoteichoic acid (transfer of D-alanine from DltB to DltD)

Locus: BSU_38499

Molecular weight: 5.90 kDa

Isoelectric point: 7.899

Protein length: 49 aa

Gene length: 150 bp

Function: D-alanylation of lipoteichoic acid

Product: membrane microprotein

Essential: -

Synonyms: ywzH

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 3,952,108  3,952,257

The protein

Catalyzed reaction/ biological activity

• interacts with both DltB and DltD and facilitates the interaction between those proteins PubMed
• involved in D-alanine transfer from DltB to DltD PubMed

Structure

• C0H3T3 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: dltX-dltA-dltB-dltC-dltD-dltE

  Description: PubMed

  Regulation

  • expression is reduced in a SigV mutant PubMed
  • the mRNA is processed between dltX and dltA by RNase Y, this requires the RicA-RicF-RicT complex PubMed

  Regulatory mechanism

  • YvrHb: activation, PubMed, in yvrHb regulon
  • Spo0A: repression, PubMed, in spo0A regulon
  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon
  • SigM: sigma factor, PubMed, in sigM regulon
  • SigX: sigma factor, PubMed, in sigX regulon
  • SigV: sigma factor, PubMed, in sigV regulon

Biological materials

Mutant

• BKE38499 (dltX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAAAAATTCCCTTCC,  downstream forward: _UP4_TTCTAATTGGAGAGAGAATA
• BKK38499 (dltX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAAAAATTCCCTTCC,  downstream forward: _UP4_TTCTAATTGGAGAGAGAATA

References

Reviews

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Original Publications

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