ywrA

168

subunit of chromate exporter (with [protein|E63704C51B53F05BAD9B35D3B6874DB9231B6D24|ywrB])

locus: BSU_36130

Molecular weight: 19.08 kDa

pI: 9.94

Protein length: 178 aa

Gene length: 537 bp

function: chromate detoxification

product: chromate exporter

essential: no

synonyms: ywrA, chr3C

List of homologs in different organisms, belongs to COG2059 (Galperin et al., 2021)

Gene

Coordinates: 3,722,005  3,722,541

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The protein

Protein family

• chromate ion transporter (CHR) (TC 2.A.51) family (with [protein|E63704C51B53F05BAD9B35D3B6874DB9231B6D24|ywrB], according to UniProt)

Structure

• [AF|O05215]

[wiki|Localization]

• cell membrane [Pubmed|22900751]

Expression and Regulation

Operons

• genes: [gene|C0BEEAD315077FE3D0D061D1D447D03E6CB48D6A|chrS]-[gene|E63704C51B53F05BAD9B35D3B6874DB9231B6D24|ywrB]-[gene|70CC5C9BCC676AB89C0BF80686BD98BA08FAE648|ywrA]

  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|C0BEEAD315077FE3D0D061D1D447D03E6CB48D6A|chrS]: repression, [Pubmed|23989926], in [regulon|protein:C0BEEAD315077FE3D0D061D1D447D03E6CB48D6A|chrS regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23989926], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A569 (ywrA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/569 NBRP B. subtilis, Japan]
• BKE36130 ([gene|70CC5C9BCC676AB89C0BF80686BD98BA08FAE648|ywrA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCCATAAATAAATAGATCG,  downstream forward: _UP4_TAGAAAAAAGCACCTGGACA
• BKK36130 ([gene|70CC5C9BCC676AB89C0BF80686BD98BA08FAE648|ywrA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CGCCATAAATAAATAGATCG,  downstream forward: _UP4_TAGAAAAAAGCACCTGGACA

References

• 19581367,22383849,22900751,23989926