ywlG

168

affects the level of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] modification

locus: BSU_36910

Molecular weight: 19.23 kDa

pI: 6.48

Protein length: 180 aa

Gene length: 543 bp

function: control of [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] modification

product: unknown

essential: no

synonyms: ywlG, ipc-33d

List of homologs in different organisms, belongs to COG4475 (Galperin et al., 2021)

Gene

Coordinates: 3,790,644  3,791,186

Phenotypes of a mutant

• inactivation suppresses the swarming defect of a [gene|248F0805272FED9B38ECBB31E2872BC9EC163CE0|ymfI] mutant [pubmed|29615499]

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The protein

Protein family

• UPF0340 family (single member, according to UniProt)

Structure

• [PDB|1V8D] (from Thermus thermophilus, 48% identity)
• [AF|P39157]

Expression and Regulation

Operons

• genes: [gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]

  description: [Pubmed|12823818]

  regulation

  • repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|12823818], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]

• genes: [gene|19829F6D993188BE854F0F61CB93777AD04CF754|ywlD]-[gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]

  description: [Pubmed|22383849]

• genes: [gene|1BAD28287257F82F907706AFD2E8FF5F3BD1B57B|ywlE]-[gene|91827FEC70F5EEB8B072D06ABA05C19FC54D5F71|ywlF]-[gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A187 (ywlG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/187 NBRP B. subtilis, Japan]
• BKE36910 ([gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTCATTCATTAAACAGC,  downstream forward: _UP4_TGAAAGGACTGCATAGCCAG
• BKK36910 ([gene|41407DE15D91959DA92799623F1364811C170F35|ywlG]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36910 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTCATTCATTAAACAGC,  downstream forward: _UP4_TGAAAGGACTGCATAGCCAG

References

• 12823818,25755103,29615499